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Fisher Scientific warmstart rtx reverse transcriptase
Warmstart Rtx Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/warmstart+rtx+reverse+transcriptase/m+mlv+reverse+transcriptase/bio_rxiv__64898__2026__01__09__696240-70-28-32
Average 86 stars, based on 1 article reviews
warmstart rtx reverse transcriptase - by Bioz Stars, 2026-09
86/100 stars

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Formulation:

Article Title: ThermiQuant™ MegaScan: High-throughput isothermal reactor with quantitative colorimetric readout for paper-based nucleic acid amplification tests
Article Snippet: To prepare 1,000 μL of 2× LAMP mix, the solution was prepared by combining 100 μL KCl (1,000 mM; Sigma-Aldrich, P9541), 160 μL MgSO 4 (100 mM; Sigma-Aldrich, M2773), 280 μL dNTP mix (10 mM; Fisher Scientific, FERR0182), 2.8 μL dUTP (100 mM; Fisher Scientific, FERR0133), 0.4 μL Antarctic Thermolabile UDG (1 U/μL; New England Biolabs, M0372S), 5.4 μL Bst 2.0 DNA polymerase (120 U/μL; New England Biolabs, M0537M), 20 μL phenol red (25 mM; Sigma-Aldrich, P3532), 100 μL Tween-20 (20%; Sigma-Aldrich, P9416), and 331.4 μL nuclease-free water (Fisher Scientific, 43-879-36). .. For diluted NP swab samples containing SARS-CoV-2, this formulation was modified to enable reverse transcription by replacing the 40 μL of RO water with an equivalent volume of WarmStart RTx Reverse Transcriptase (Fisher Scientific, 43-879-36). .. To prepare 200 μL of the LAMP final master mix, 125 μL of the 2× LAMP solution was combined with 25 μL 10× primer mix (16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LF/LB; final concentrations 1.6 μM FIP/BIP, 0.2 μM F3/B3, 0.4 μM LF/LB; see Table S6), 0.67 μL Bst 2.0 DNA polymerase, 1 μL betaine (5 M; Sigma-Aldrich, B03005VL), 3.13 μL bovine serum albumin (BSA) (40 mg/mL; Sigma-Aldrich, A2153), 36.0 μL trehalose (1.75 M; Thermo Scientific Chemicals, 182550250), and 9.2 μL nuclease-free water.

Modification:

Article Title: ThermiQuant™ MegaScan: High-throughput isothermal reactor with quantitative colorimetric readout for paper-based nucleic acid amplification tests
Article Snippet: To prepare 1,000 μL of 2× LAMP mix, the solution was prepared by combining 100 μL KCl (1,000 mM; Sigma-Aldrich, P9541), 160 μL MgSO 4 (100 mM; Sigma-Aldrich, M2773), 280 μL dNTP mix (10 mM; Fisher Scientific, FERR0182), 2.8 μL dUTP (100 mM; Fisher Scientific, FERR0133), 0.4 μL Antarctic Thermolabile UDG (1 U/μL; New England Biolabs, M0372S), 5.4 μL Bst 2.0 DNA polymerase (120 U/μL; New England Biolabs, M0537M), 20 μL phenol red (25 mM; Sigma-Aldrich, P3532), 100 μL Tween-20 (20%; Sigma-Aldrich, P9416), and 331.4 μL nuclease-free water (Fisher Scientific, 43-879-36). .. For diluted NP swab samples containing SARS-CoV-2, this formulation was modified to enable reverse transcription by replacing the 40 μL of RO water with an equivalent volume of WarmStart RTx Reverse Transcriptase (Fisher Scientific, 43-879-36). .. To prepare 200 μL of the LAMP final master mix, 125 μL of the 2× LAMP solution was combined with 25 μL 10× primer mix (16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LF/LB; final concentrations 1.6 μM FIP/BIP, 0.2 μM F3/B3, 0.4 μM LF/LB; see Table S6), 0.67 μL Bst 2.0 DNA polymerase, 1 μL betaine (5 M; Sigma-Aldrich, B03005VL), 3.13 μL bovine serum albumin (BSA) (40 mg/mL; Sigma-Aldrich, A2153), 36.0 μL trehalose (1.75 M; Thermo Scientific Chemicals, 182550250), and 9.2 μL nuclease-free water.

Reverse Transcription:

Article Title: ThermiQuant™ MegaScan: High-throughput isothermal reactor with quantitative colorimetric readout for paper-based nucleic acid amplification tests
Article Snippet: To prepare 1,000 μL of 2× LAMP mix, the solution was prepared by combining 100 μL KCl (1,000 mM; Sigma-Aldrich, P9541), 160 μL MgSO 4 (100 mM; Sigma-Aldrich, M2773), 280 μL dNTP mix (10 mM; Fisher Scientific, FERR0182), 2.8 μL dUTP (100 mM; Fisher Scientific, FERR0133), 0.4 μL Antarctic Thermolabile UDG (1 U/μL; New England Biolabs, M0372S), 5.4 μL Bst 2.0 DNA polymerase (120 U/μL; New England Biolabs, M0537M), 20 μL phenol red (25 mM; Sigma-Aldrich, P3532), 100 μL Tween-20 (20%; Sigma-Aldrich, P9416), and 331.4 μL nuclease-free water (Fisher Scientific, 43-879-36). .. For diluted NP swab samples containing SARS-CoV-2, this formulation was modified to enable reverse transcription by replacing the 40 μL of RO water with an equivalent volume of WarmStart RTx Reverse Transcriptase (Fisher Scientific, 43-879-36). .. To prepare 200 μL of the LAMP final master mix, 125 μL of the 2× LAMP solution was combined with 25 μL 10× primer mix (16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LF/LB; final concentrations 1.6 μM FIP/BIP, 0.2 μM F3/B3, 0.4 μM LF/LB; see Table S6), 0.67 μL Bst 2.0 DNA polymerase, 1 μL betaine (5 M; Sigma-Aldrich, B03005VL), 3.13 μL bovine serum albumin (BSA) (40 mg/mL; Sigma-Aldrich, A2153), 36.0 μL trehalose (1.75 M; Thermo Scientific Chemicals, 182550250), and 9.2 μL nuclease-free water.



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